S.B. [Biology] (Massachusetts Institute of Technology), S.B. [Chemical Engineering] (Massachusetts Institute of Technology), M.S. (Washington University), Ph.D. [Biomedical Engineering] (Washington University), Post-Doctoral Fellowship [Bioengineering and Chemical Engineering] (University of California)
Associate Professor, Mechanical Engineering and Division of Medical Sciences, University of Victoria
Member, Centre for Advanced Materials & Related Technology
Research InterestsBiomaterials; Regeneration; Scaffold; spinal cord injury; Stem cell; Tissue engineering
Dr. Willerth may be accepting summer students for 2016. See Current Opportunities in the Lab (below).
How can we restore function to diseased or damaged tissue? The field of tissue engineering can meet this demand by producing replacements for tissue and organs that no longer function properly. Regenerating simple tissues or repairing defects, such as in skin, can be accomplished using biomaterial scaffolds alone. The current challenge is how to develop engineered tissues for replacing more complex organs such as the spinal cord. Dr. Willerth’s research program addresses this challenge and its complexities. These complexities include finding how to replace the variety of cell types present in tissue while also achieving the necessary arrangements of these cells. In particular, her work has focused on the use of pluripotent stem (iPS) cells, which can differentiate into any type of cell found in the body. Her research group analyzes the behaviour of stem cells inside three-dimensional biomaterial scaffolds with the goal of engineering complex tissues.
Dr. Willerth is an Assistant Professor of Biomedical Engineering at the University of Victoria. She is also a member of the Centre for Advanced Materials & Related Technology and a Principal Investigator at ICORD. She obtained her S.B. in biology and another in chemical engineering from the Massachusetts Institute of Technology. Her M.S. and Ph.D. in biomedical engineering were completed at Washington University and she pursued her NIH supported Post-Doctoral Fellowship in bioengineering and chemical engineering at the University of California.
When cells are transplanted into the harsh environment of injured tissue very few survive. Dr. Willerth’s research works to make scaffolds which can increase the survival of these cells. The biomaterial scaffolds can be used in combination with stem cell therapies as a potential strategy for regeneration and improving functional recovery.
Dr. Willerth enjoys working at ICORD because it enables her to work with a variety of scientists, each with their own unique perspective on how to address the challenges associated with SCI. Her lab is located in the University of British Columbia Faculty of Medicine campus on Vancouver Island, where she shares space with other biomedical researchers. She appreciates the shared lab space because it allows “real interdisciplinary research.”
Dr. Willerth collaborates with Martin Jun of the Department of Mechanical Engineering at the University of Victoria on producing electrospun scaffolds.
She works with MedGenesis Therapeutix Inc. on ways of delivering the protein GDNF, which increases the survival of neurons.
She also works with Stem Cell Technologies products to engineer neural tissue in a reproducible fashion.
Fibrin is a protein involved in the clotting of blood. It is commonly used clinically as surgical glue. As a graduate student, Dr. Willerth was the first to determine the conditions needed to grow embryonic cells in fibrin scaffolding. Her work with three-dimensional fibrin scaffolds involves environments which more closely resemble the human body, allowing better understanding of how cell-tissue processes work. For example, her research has shown how naturally occurring proteins and hormones can change stem cells into recovery cells.
Techniques employed in the lab:
- Flow cytometry
- Three-dimensional cell culture systems
- Quantitative real time polymerase chain reaction (qPCR)
Affiliation with organizations and societies:
- Centre for Advanced Materials and Technology (CAMTEC), University of Victoria
- Centre for Biomedical Research (CBR), University of Victoria
- Biomedical Engineering Society (BMES)
- Board of Directors, Canadian Biomaterials Society
Some of Dr. Willerth’s recent major awards and accomplishments include:
- Young Innovator in Cellular and Molecular Bioengineering (Biomedical Engineering Society, 2015)
- Faculty of Engineering Award for Teaching (University of Victoria, 2015)
- Canada Research Chair in Biomedical Engineering (2013)
- Post-doctoral Fellowship (National Institutes of Health, 2010)
- Travel Awards (Biomedical Engineering Society, 2007)
Current Lab Members
Current Opportunities in the Lab
Yes. Students are encouraged to email Dr. Willerth directly to find out more about opportunities. Dr. Willerth may be accepting summer students for 2016 for paid positions. She prefers students with tissue culture experience.
- Edgar, JM, Robinson, M, Willerth, SM. 2017. Fibrin hydrogels induce mixed dorsal/ventral spinal neuron identities during differentiation of human induced pluripotent stem cells.. Acta Biomater. doi: 10.1016/j.actbio.2017.01.040.
- Willerth, SM. 2016. Engineering personalized neural tissue using functionalized transcription factors.. Neural Regen Res. doi: 10.4103/1673-5374.193229.
- Agbay, A et al.. Biomaterial Strategies for Delivering Stem Cells as a Treatment for Spinal Cord Injury.. Cells Tissues Organs (Print). doi: 10.1159/000446474.
- Robinson, M, Chapani, P, Styan, T, Vaidyanathan, R, Willerth, SM. 2016. Functionalizing Ascl1 with Novel Intracellular Protein Delivery Technology for Promoting Neuronal Differentiation of Human Induced Pluripotent Stem Cells.. Stem Cell Rev. doi: 10.1007/s12015-016-9655-7.
- Montgomery, A, Wong, A, Gabers, N, Willerth, SM. 2015. Engineering personalized neural tissue by combining induced pluripotent stem cells with fibrin scaffolds.. Biomater Sci. doi: 10.1039/c4bm00299g.